Production of Ulvan Oligosaccharides with Antioxidant and Angiotensin-Converting Enzyme-Inhibitory Activities by Microbial Enzymatic Hydrolysis

Seaweed oligosaccharides have attracted attention in food, agricultural, and medical applications recently. Compared to red brown seaweeds, fewer studies focused on the biological activity of green seaweed’s oligosaccharides. This study aimed produce bioactive ulvan via enzymatic hydrolysis from seaweed Ulva lactuca. Ulvan, a water-soluble polysaccharide, was obtained by hot water extraction. Two isolated marine bacteria, Pseudomonas vesicularis MA103 Aeromonas salmonicida MAEF108, were used multiple hydrolases, such as ulvanolytic enzymes, amylase, cellulase, xylanase, degrade extract. An ultrafiltration system separate hydrolysate acquire (UOS). The characteristics extract UOS determined yield, reducing sugar, uronic acid, sulfate group, total phenols. FT-IR spectrum indicated that presented bands associated with O-H, C=O, C-O, S=O stretching. Angiotensin I converting enzyme (ACE) inhibition antioxidant activities vitro evaluated UOS. These results provide practical approach producing microbial can benefit development seaweed-based products at industrial scale.